Respiratory system
Lung
Look at slide 48. The differential staining of this section
shows the amount and distribution of elastic and collagenous fibers, smooth muscle,
basement membranes, epithelium, cartilage, blood vessels, pleura, alveolar macrophages and
capillary walls. The alveoli are collapsed in many regions. So, this slide is
best for the bronchi and associated structures. Look at the Pleural covering
which shows a red-colored mesothelial lining. This is
supported by connective tissue containing abundant collagen fibers stained green.
Bronchus
Find a bronchus in slide 48. Study its epithelium which is ciliated,
respiratory epithelium with Goblet cells. The epithelium rests on a
purple-stained Basement Membrane.
Find the smooth muscle in the wall of the bronchus. The fibers are
arranged circularly and stained pink. Prominent features of the bronchial wall are the
cartilaginous bars and plates. You can also see Bronchial vessels and nerve fiber
bundles. Bronchi are accompanied by branches of the Pulmonary arteries. Note
that the arteries and veins contain a considerable amount of elastic fibers.
Look at the epithelium under a higher power. The pseudostratified
characteristic continues. Also, you may see Goblet cells. Note the thick
purple stained basement membrane under which is a layer of black-stained, mostly
longitudinally arranged elastic fibers.
Look at slides 46 and 47 for more structures in the lung. The lung section
in slide 46 is partially covered by Pleura. Slide 46 is good for a study of
air passages smaller than bronchi. These include Terminal Bronchioles, Respiratory
Bronchioles, Alveolar Ducts, Alveolar Sacs and Alveoli. Find each of these structures on
your slide 46, using the following photos as a guide.
The larger bronchioles are lined with an epithelium that is
pseudostratified columnar with or without cilia. However, the Goblet cells are no longer
present. Also, there are no more cartilage plates. As the bronchioles become
smaller, the epithelium becomes cuboidal.
Most bronchioles show a pronounced smooth muscle coat. In this
slide, the smooth muscle is red, embedded in green connective tissue. The
bronchioles are also accompanied by pulmonary arteries, seen in the photograph to the
left. Note that the bronchiole is easily distinguished from the thin walled alveoli.
As you study these sections, you can appreciate the extent of the
capillary bed in the lung. All blood vessels are filled with red blood cells, which
delineate the capillary spaces. Occasional blood cells have spilled out....this is a
fixation or postmortem artifact.
This photograph shows a higher magnification of the wall of a
bronchiole. You can see the capillaries as well as the smooth muscle in the
wall. This is a small bronchiole because its epithelium is more cuboidal.
A higher magnification of a bronchiole wall shows the ciliated cuboidal
epithelial cells. There are also some non-ciliated rounded secretory cells called
Clara Cells. These are distinguished by their smooth rounded apical
"domes" which project into the bronchiolar lumen. Note again the smooth muscle
in the wall of the bronchiole. What is the function of the Clara cell?
Find both terminal bronchioles and respiratory bronchioles in this
section. Recall that respiratory bronchioles have outpocketings of alveoli in the wall
(hence their name). Terminal bronchioles, such as the one shown in the photograph to the
left, end and connect with the walls of alveoli. In this photograph, the alveoli are
arranged in a long duct and then they expand to form an alveolar sac. Find the same
structures on your slide.
A higher magnification of a terminal bronchiole shows the transition
between the bronchiolar wall and the alveolar walls.
Look at the wall of alveoli. The Type I alveolar
cells are the extremely thin (0.2 microns) squamous cells. Sometimes, only their
nuclei are visible with the light microscope.
Another type of alveolar cell is the Type II cell.
These are characterized by a reddish, foamy or vacuolated cytoplasm. Usually they
are located at the angular junctions of alveolar walls.
Two more Type II cells are shown in the photograph to the left. The
photograph beneath it shows more examples of the alveolar wall. What is the function
of the Type II Alveolar cells?
Look at Slide 47. In this slide you should be able to see small Bronchi.
You can also see bronchioles which are distinguished by the absence of cartilage
plates. Identify terminal and respiratory bronchioles, alveolar ducts and alveolar sacs.
Throughout the section, you may see particle-laden alveolar macrophages (shown in
the following photograph).
Why do the ciliated cells extend further down the bronchiolar tree than
the Goblet cells?
As you study the respiratory system, draw the cellular and
connective tissue layers which separate blood from the air spaces. Also, be
able to trace the blood supply to and from the lung and heart.
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