Graduate Microanatomy, 1998
Graduate Microanatomy, 1998
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Lab Exercises: Collecting ducts, calyces, Ureter and Bladder
Date page was last edited 07/19/01 |
Laboratory Exercises: Kidney Glomerulus Look at the Masson stained slide of the kidney and the H & E stained section. Hold it up to the light to distinguish the CORTEX from the MEDULLA. In the Masson stained section, note the blue-stained CAPSULE covering the surface. View the same regions with the low magnification of the microscope.
The outer regions, called the cortex are distinguished by numerous sectioned profiles of tubules and glomeruli. Identify the capsule and look at a glomerulus with a higher magnification. It is essentially a tuft of capillaries embedded in a double-walled epithelium (like a fist in a balloon). The epithelium is called "Bowman's capsule". That part of the epithelium covering the capillaries is called the visceral epithelium. The part forming the capsule is called "parietal epithelium". The filtration membrane is formed from the endothelial cells of the capillaries, basement membrane, and visceral epithelium. 1. What cell types are found in the visceral epithelium?
2. Draw the epithelial cell that forms the filtration membrane or barrier.
The above photograph shows a higher magnification of the glomerulus in Bowman's capsule. Note the capillaries in the glomerulus. The space between the two epithelia is called Bowman's space. 3. Draw an arrow showing the route and direction of the urinary filtrate across the filtration membrane.
4.Where does the filtrate go after it passes the filtration membrane?
This photograph is from Hemotoxylin and Eosin stained kidney slide. Look at glomeruli in this slide as well and identify the capillaries, Bowman's capsule and Bowman's space. The afferent arteriole is the source of the capillaries in the Glomerulus. It is shown in this photograph, although the smooth muscle cells are difficult to see. The site where the arteriole enters the Renal corpuscle is called the "Vascular Pole". The opposite end is continuous with the proximal convoluted tubule. The above micrograph shows cells of the Proximal tubule beginning and joining with the cells of the Bowman's capsule (parietal epithelium). This is called the Urinary Pole. 4. Find the vascular pole and the urinary pole on the above photo and following photographs. Also, find examples in your slide set.
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The above photograph shows a higher magnification of the Urinary pole. It shows the transition between Bowman's Space in the Renal Corpuscle and the Proximal Convoluted tubule. Note that the cells show an abrupt transition from simple squamous to simple cuboidal in the proximal tubule (PT). Note also that the connective tissue elements supporting the tubules are stained bright blue in this Masson stained section.
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