This transmission electron micrograph to the right shows the microtubules in longitudinal ultrathin section. Note, the tubulin molecules cannot be visualized in this preparation.

Early electron microscopists found that in order to preserve microtubules, they had to fix the cells in glutaraldehyde at room temperature. Why do you think the temperature conditions were important? What might happen if they fixed the cells for 30 min in the cold?

The first stage of formation is called "nucleation". The process requires tubulin, Mg++ and GTP and also proceeds at 37 C. This stage is relatively slow until the microtubule is initially formed. Then the second phase, called "elongation" proceeds much more rapidly.

During "nucleation", an alpha and a beta tubulin molecule join to form a heterodimer. Then these attach to other dimers to form oligomers which elongate to form protofilaments. Each dimer carries two GTP molecules. However the GTP that appears to function binds to the beta tubulin molecules. When a tubulin molecule adds to the microtubule, the GTP is hydrolyzed to GDP. Eventually the oligomers will join to form the ringed microtubule. The hydrolysis of GTP of course is facilitated at a temperature of 37 C and stopped at temperatures of 4 C.

This figure shows that, as the oligomers assemble, they form a series of rings, 25 nm in diameter. In cross section, each ring consists of 13 beads. The rows of beads in longitudinal section are called protofilaments.

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In the cell itself, microtubules are formed in an area near the nucleus called the "aster". Microtubules are polar with a plus end (fast growing) and a minus end (slow growing). Usually the minus end is the anchor point. In this figure, the plus end is shown to the left by the numerous tubulin dimers. This is the end that carries the GTP molecules which may be hydrolyzed to GDP. Hydrolysis is not necessary, however (see p 810 in text and discussion below).

Tests have shown that microtubules will form normally with nonhydrolyzable GTP analog molecules attached. However, they will not be able to depolymerize (see below). Thus, the normal role of GTP hydrolysis may be to promote the constant growth of microtubules as they are needed by a cell.
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As described in your text, the cell can provide a GTP cap on the growing end of a microtubule to regulate further growth. This happens when the tubulin molecules are added faster than the GTP can be hydrolyzed. Thus, the microtubule becomes stable and does not depolymerize. It may also be encouraged to continue growing. Once the GTP is hydrolyzed, it begins to shrink, however. Another way of capping a microtubule is to put a structure at its end, such as a cell membrane.

Furthermore, it is believed that some of these MAPs may bind to special sites on the alpha tubulin that form after it is in the microtubule. These are sites where a specific molecule is acetylated or the tyrosine residue is removed from the carboxy terminal. These sites are important marker sites for stabilized microtubules, because they disappear when microtubules are depolymerized.

This figure shows a 3-D view of a neuron with its processes containing microtubules. At higher magnifications, the vesicles are seen attached to MAPs and moving along the microtubule conveyer belt. The MAPs include kinesins and dynein which "walk" along the microtubules in opposite directions.The kinesins move the vesicle along towards the plus end and dynein walks towards the minus end. In neurons, as the microtubules grow from the cell body through the processes, the plus end is more peripheral. These proteins have head regions that bind to microtubules and also bind ATP. The head domains are thus ATPase motors. The tail domain binds to the organelle to be moved. It is not known how the energy from ATP breakdown is converted into vectorial transport.

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One can label beads with kinesin or dyneins and watch the direction of movement in a cell at the light microscopic level. What would happen if the beads were simply labeled with "cytoplasmic extract"? This cartoon shows the motility process in vitro. The tubule is moving along a negatively charged glass surface and the vesicle moves along the tubule.

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