Molecular Biology Core Facility

The Marine Biomedical Institute/Anatomy and Neurosciences Molecular Biology Facility at the University of Texas Medical Branch provides equipment and advice for investigators, postdocs, and their students planning to utilize molecular biological techniques in their research. It is directed by Gregg T. Nagle, Ph.D. (shown in the photograph)

Number of visitors to this site beginning 08/17/97

Location

Medical Research Building, room 2.118 in the Department of Anatomy and Neurosciences.

Eligible Users

Faculty of Marine Biomedical Institute and Department of Anatomy and Neurosciences and their collaborators

Contact Information

Gregg T. Nagle, Ph.D., Department of Anatomy and Neurosciences, Medical Research Building 10.104, University of Texas Medical Branch; Mail Route 1069; email gtnagle@utmb.edu

Types of services and support available

Technical expertise is provided for most commonly used techniques including DNA and RNA isolation, cDNA synthesis, PCR, Northern and Southern blot analyses, RNAse protection assays, cDNA library construction and screening, DNA cloning, electroporation, DNA sequencing and baculovirus protein expression. Expertise in peptide purificiaton using RP-HPLC is also available. Investigators provide their own consumable supplies.

Facility Instrumentation and Capacity:

The facility occupies approximately 550 sq ft of floor space. Its capital equipment includes:

DNA thermal cyclers (2)
Beckman HPLC
UV crosslinker
bacteriological incubators
CO2/O2/N2 incubators
orbital shaking incubator
hybridization ovens (2)
laminar flow hood
water baths
DNA and RNA transfer apparatus, power supplies
gel dryer
electroporator
sonic dismembranator
Milli-Q PF water purification system
DyNA Quant 200 fluorometer
Beckman L8-80M ultracentrifuge
Beckman J2-21 centrifuge
microfuges (2)
lyophilizer
Savant Speed-Vac concentrators (2)

Cell Biology faculty using molecular biology techniques in their own laboratories

Cheryl S. Watson, Ph.D.
Regino Perez-Polo, Ph.D.
Jane Bottenstein, Ph.D.
KyungSoon Chung, Ph.D.
Guilio Taglialatela, Ph.D.
Chandra Yallamapalli, D.V.M., Ph.D.
Pomila Singh, Ph.D.
Lisa A. Elferink, Ph.D.
Jin Mo Chung, Ph.D.
Soon Chung, Ph.D.
Ping Wu, Ph.D.

and Internet guidelines.

Last updated: 08/15/03